Extended Data Fig. 6: Incorporation of L-LA into TCA intermediates.
From: Lactate limits CNS autoimmunity by stabilizing HIF-1α in dendritic cells
a, mRNA expression of shown genes in BMDCs isolated from WT mice and stimulated with LPS and mitoPQ or L-LA for 6 h (n = 3–4 per group). b, mRNA expression of shown genes in BMDCs isolated from WT mice and treated with mitoPQ for 6 h (n = 4–5 per group). c, 13C incorporation into TCA intermediates in BMDCs isolated from WT mice and treated with uniformly labelled 13C-lactate (L-LA*), L-LA or LPS for 1 h (n = 3 per group). d, Pyruvate intracellular levels in WT BMDCs after treatment with LPS, L-LA or D-LA for 1 h (n = 4 per group). e,f, mtROS production (e) and mRNA expression of shown genes (f) in WT BMDCs pre-treated with LDH-inhibitor oxamate, L-LA or LPS (n = 3–4 per group). g, mRNA expression of shown genes in splenic DCs isolated from WT or HIF-1αItgax mice then treated with LPS or pyruvate for 6 h (n = 3–4 per group). Statistical analysis was performed using one-way ANOVA with Tukey’s, Šídák’s or Dunnett’s post-hoc test for selected multiple comparisons for a,e–g and unpaired Student’s t-test for b. Data shown as mean ± s.e.m.
