Extended Data Fig. 1: Analysis of HIF-1Itgax mice during EAE.
From: Lactate limits CNS autoimmunity by stabilizing HIF-1α in dendritic cells
a, Uniform manifold approximation and projection (UMAP) displaying CNS DCs analysed by scRNA-seq during EAE. b, GSEA of hypoxia activation in DC subsets (cDC1, cDC2 and pDC) from scRNA-seq dataset53. c,d, Representative dot plot (c) and flow cytometry analysis (d) of HIF-1α expression in splenic cDC1s (CD8+CD11b−), cDC2s (CD8−CD11b−) and pDCs (B220+) 25 days after EAE induction in WT mice (n = 5 mice per group). e, Gating strategy used to analyse CD4+ T cells in the CNS of mice subjected to EAE. f, IFNγ+, IFNγ+IL-17+ CD4+ T cells in spleens of WT (n = 5) and HIF-1αItgax (n = 4) mice subjected to EAE. g,h, Cytokine production (20 μg/mL MOG33–55) (g) and proliferative recall response to ex vivo MOG35–55 restimulation (h) of splenocytes isolated from mice from (f (n = 3 for WT IFNγ, n = 4 for HIF-1αItgax IFNγ, IL-17 and WT GM-CSF, n = 5 otherwise). i, Absolute number of DCs in CNS from WT and HIF-1αItgax mice (n = 5 mice per group). j–l, Heat map (j), IPA (k) and GSEA analysis (l) of RNA-seq of DCs isolated from the CNS of WT or HIF-1αItgax mice subjected to EAE. Statistical analysis was performed using unpaired Student’s t-test for f and g and two-way ANOVA followed by Šídák’s multiple comparisons test for h. Data shown as mean ± s.e.m.
