Extended Data Fig. 8: Tumor progression through STING-dependent ER stress response.
From: Non-cell-autonomous cancer progression from chromosomal instability
a, Relative expression levels of Ifnb1 and ISGs in mock, cGAMP, or Poly(I:C)-transfected 4T1 cells; bars represent mean ± SD, two-way ANOVA test, n = 3 independent experiments (n = 2 for Poly(I:C)-treated), each with two technical replicates. b-c, Immunoblots for BiP and STING of WT and Sting KO cells of B16F10, CT26 (b) and EO771.LMB (c) with β-actin (b) or COX-IV (c) as loading control. d, Cellular growth curves for of Control and IRE1α (Ern1), PERK (Eif2ak3), or ATF6 (Atf6)-KO 4T1 cells; data are presented as mean values ± SD., n = 3 per condition. e, Immunoblots of Control and IRE1α (Ern1), PERK (Eif2ak3), or ATF6 (Atf6)-KO 4T1 cells blotted for IRE1a, PERK, ATF6, and α-tubulin as a loading control. f, Relative expression levels of Ccl2, Cxcl1, and Il11 in tumor cells isolated from primary tumors resected on day 7; bars represent mean values ± SD, * p < 0.05, ** p < 0.01, two-sided t-test, n = 4 animals per group. g, Number of surface lung metastasis in mice inoculated with control 4T1 cells or cells lacking cytokines (left) or Sting1-depleted cells or Sting1-depleted cells overexpressing cytokines (right); bars represent the median, ** p < 0.01, two-sided Mann-Whitney test, n = 10 animals per group.
