Extended Data Fig. 11: Chronic cGAS–STING activation forebodes poor prognosis in human TNBC.
From: Non-cell-autonomous cancer progression from chromosomal instability
a, Immunoblots for STING in IMR90 after the first and fifth cGAMP stimulation with β-actin as loading control. b, Normalized STING protein levels in control, Cgas KO, and Sting1 KO cells; bars represent mean ± SD, * p < 0.05, two-sided ratio-paired t-test, n = 3 independent experiments. c, Immunoblots for STING of 4T1 WT and Cgas KO cells treated with BafA1 or vehicle in the presence of translation inhibitor cycloheximide with β-Actin as loading control. d, Representative high-resolution image of human tumor sample stained with DAPI (DNA) and anti-cGAS antibody showing selective localization of cGAS at micronuclei, scale bar 5-μm. e, Bar graph depicting the relationship between tumor cGAS and STING protein levels in TNBC, two-sided Chi-Square χ2-test, n = 179 tumors. f-g, Distant metastasis-free survival (DMFS) of patients with TNBC stratified based tumor STING (f) and cGAS (g) expression intensity, log-rank test, n = 155 patients. h, Percentage of tumor infiltrating lymphocytes in TNBC tumors stratified based on protein expression of cGAS and STING, bars represent mean ± s.e.m, n = 16 and 57 patients in the cGASlowSTINGhigh and cGAShighSTINGlow tumors, respectively, two-sided t-test.
