Fig. 3: The TMX1–DIESL enzymatic complex drives alternative triglyceride synthesis.
From: Identification of an alternative triglyceride biosynthesis pathway
a, Immunoblot analysis of TMX1 and haemagglutinin (HA)-tagged DIESL (3×HA–DIESL) in HAP1 cells lacking endogenous DIESL (and TMX1), with or without crosslinking by 1% paraformaldehyde (PFA). Red arrowheads indicate the DIESL–TMX1 heterodimer, and the asterisk indicates a non-DIESL band. b, Co-immunoprecipitation of TMX1 with DIESL from rescued HAP1 cells (the asterisk indicates antibody chains). CANX, calnexin; PDI, protein disulfide isomerase; EIF4G, eukaryotic translation initiation factor 4G. c, Sequence conservation of catalytic dyads composed of a histidine (H, blue asterisk) and an aspartate (D, red asterisk) across acyltransferases (Uniprot accession numbers in parentheses). Species are Homo sapiens, Caenorhabditis elegans, Arabidopsis thaliana, Saccharomyces cerevisiae, Mycobacterium leprae, Chlamydia trachomatis, Escherichia coli and Thermotoga maritima. d, Western blot of HAP1 cells rescued with WT or catalytic-dead (H130A) DIESL (the asterisk indicates a non-specific band). FASN, fatty acid synthase. e, Analysis of lipid droplets (BODIPY 493/503 fluorescence intensity) in DIESL-rescued HAP1 cells by flow cytometry.
