Fig. 3: DMAPP and IPP function as molecule glues to activate γδ T cells.
From: Phosphoantigens glue butyrophilin 3A1 and 2A1 to activate Vγ9Vδ2 T cells
a, ITC analysis indicates that DMAPP and IPP promote the association between BTN3A1 B30.2 and BTN2A1 B30.2. b, Cartoon model of the BTN3A1 B30.2–DMAPP–BTN2A1 B30.2 complex (PDB: 8JYC). DMAPP is shown as a stick model and a water molecule is shown as a red sphere. c, TNF release by Vγ9Vδ2 T cells in response to zoledronate stimulation of BTN3A1+CD80+ CHO-K1 cells (left; n = 6) or BTN2A−/− MIA PaCa-2 (BTN2A1/BTN2A2 KO) cells (right; n = 5, representative of four independent experiments) transfected with the plasmids for the indicated BTN2A1 mutants. Residues in BTN2A1 B30.2 that directly interact with DMAPP and residues in BTN2A1 B30.2 that directly interact with Trp350/Trp391 in BTN3A1 B30.2 are indicated. Statistical analysis was performed using Welch’s analysis of variance (ANOVA) with Dunnett’s T3 multiple-comparison test, comparing each BTN2A1 mutant with the WT control. Data are mean ± s.e.m. d, TNF release by Vγ9Vδ2 T cells in response to zoledronate-treated (Zol, 10 µM) BTN2A−/− MIA PaCa-2 cells (n = 6, representative of four independent experiments) that were transfected with plasmids encoding the indicated BTN2A1 mutants (based on the analysis in Extended Data Fig. 3d). Statistical analysis was performed using Welch’s ANOVA with Dunnett’s T3 multiple-comparison test, comparing with the WT control. Data are mean ± s.e.m.
