Extended Data Fig. 5: P1 nuclease degradation of reaction products. | Nature

Extended Data Fig. 5: P1 nuclease degradation of reaction products.

From: Antiviral type III CRISPR signalling via conjugation of ATP and SAM

Extended Data Fig. 5

HPLC analysis of nuclease P1-mediated hydrolysis reactions towards Cmr’s products (left). cA2 (cyclic di-3′,5′-adenylate) and pApA (5′- phosphoadenylyl- (3′ → 5′)- adenosine) were used as controls. The proposed reactions of P1 were shown on the right. Uncropped HPLC traces are available in Supplementary Data Fig. 6.

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