Extended Data Fig. 7: pppApA synthesis by the wild-type and variant BfrCmr complexes. | Nature

Extended Data Fig. 7: pppApA synthesis by the wild-type and variant BfrCmr complexes.

From: Antiviral type III CRISPR signalling via conjugation of ATP and SAM

Extended Data Fig. 7

In vitro pppApA synthase activity of wt and variants B. fragilis Cmr, analysed by HPLC following incubation of 2 µM Cmr with 0.5 mM ATP for 30 min. The D70N/E151R double mutant synthesises pppApA but not SAM-AMP. Uncropped HPLC traces are available in Supplementary Data Fig. 7.

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