Extended Data Fig. 1: Plasmid challenge assay.
From: Antiviral type III CRISPR signalling via conjugation of ATP and SAM
a, Plasmid pBfrCmr1-6 was constructed by Gibson assembly to express genes cmr1–cmr6 (junctions indicated by black squares); Plasmid pCRISPR was constructed to express the CRISPR array along with Cas6. Plasmid maps were generated by Benchling (https://benchling.com/). b, Different variants of ancillary effectors were tested in the B. fragilis Cmr wild type target (tetR) and non-target (pUC19) system. CorAtr is the truncated CorA (aa 1-428) and NrNΔ is an inactive variant of NrN (D85A/H86A/H87A). The schematic shows cartoons of the three plasmids used in these experiments. c, The ancillary effectors CorA and NrN were assayed in the Mycobacterium tuberculosis (Mtb) Cmr wild type system. TsuCsx1 from Thioalkalivibrio sulfidiphilus (activated by cA4) acted as a positive control. Uncropped images and gels are available in Supplementary Data Fig. 4.
