Extended Data Fig. 6: Resistance markers whole-genome analysis of parental and hybrid lines.

Schematic display of an artificial chromosome containing arbitrary loci of the three antibiotic resistance genes (Hygromycin – HYG, Blasticidin – BSD, Nourseothricin – SAT) used as parental markers in this study. (a) Leishmania major F1 hybrids. (b) L. major backcross hybrids. (c) L. tropica F1 hybrids. Raw reads were processed and displayed using Geneious prime software v2021.2.2. Upper case letters in green provide a unique identifier for each Leishmania parental or hybrid. Red abbreviations present on first panel of (a) are applicable to all panels in the figure: Cov, coverage; r-Chr, artificial resistance chromosome; A-r, aligned reads. Panel I was repeated from (a) to (b) for illustrative purposes as this was the selected F1 hybrid for backcrosses.