Extended Data Fig. 7: Whole-genome analysis of Leishmania major hybrids.

(a, b) Allele-specific chromosomal copy number as inferred by read depth and allelic proportions. Biparental ancestry was confirmed across the whole genome for crosses between parentals 1×2 and backcrosses of (1×2) F1 hybrid with parental 2′ or parental 2″ (a) and a parental 1×3 cross (b). Biparental inheritance of backcrosses for 1×2 crosses are also shown in Fig. 5d. Upper case letters provide a unique identifier for each Leishmania parental or hybrid. (2×3) crosses not present because within strains there were very few fixed differences between the parents, and therefore not enough informative sites to call ancestry. Parental 1 plot was repeated from (a) to (b) for illustrative purposes as this parental composed both F1 crosses setup. (c) Somy heatmap of sequenced Leishmania major parentals and hybrids. Whole-genome analysis shows characteristic Leishmania major aneuploidy chromosome distribution in all samples. (a-c) Parental 1, WR-SSU-HYG; Parental 2, FVI-FKP40-BSD; Parental 3, FVI-FTL-SAT. Parental 2′, FV1-FKP40-SAT; Parental 2″, FV1-SSU-SAT. Upper case letters provide a unique identifier of each Leishmania parental or hybrid.