Extended Data Fig. 2: IgM promotes Leishmania hybrid formation in vitro.
From: Leishmania genetic exchange is mediated by IgM natural antibodies
(a) Workflow for in vitro crossing of Leishmania parental lines (L. major or L. tropica). Mating wells were prepared with 50 µg/mL IgM in complete Schneider’s media. An improved detailed step-by-step procedure to maximize IgM-induced Leishmania major mating in vitro is available at https://doi.org/10.21203/rs.3.pex-2345/v1. (b) Data summary of hybrid formation from in vitro crossing of L. major parental lines: Parental 1, WR-SSU-HYG; Parental 2, FVI-FKP40-BSD; parental 3, FVI-FTL-SAT. L. major hybrids genotyping by PCR targeting parental selectable drug markers available at Fig. 1h. (c) Data summary of hybrid formation from in vitro crossing of L. tropica parental lines: Parental 4, K27-SSU-HYG; Parental 5, K27-SSU-SAT. (d) L. tropica hybrids genotyping by PCR targeting parental selectable drug markers HYG, and SAT. Parental 4, K27-SSU-HYG; Parental 5, K27-SSU-SAT; ntc, no template control; L, 1kb-plus ladder.
