Extended Data Fig. 9: Development of antigen-specific CARs for neuroblastoma antigens.
From: Targeting of intracellular oncoproteins with peptide-centric CARs
a. ELISA of PHOX2B scFv A7 using PHOX2B p/MHC and decoy peptide on HLA-A*24:02. b. Schematic of second-generation CAR constructs. c. A7 CAR transduced into primary CD8 cells binds PHOX2B dextramer but not HLA-A*02:01 dextramer. d. A7 CAR preferentially binds PHOX2B dextramer but cross-reacts with mismatched peptides PBK and CHRNA3 on HLA-A*24:02 at high affinity. e. A7 CAR potently kills HLA-A*24:02 neuroblastoma lines, but also kills HLA+/antigen− tumor cells. f. Tetramer and dextramer gating strategy for pMHC staining.
