Extended Data Fig. 10: PHOX2B A7 CAR.
From: Targeting of intracellular oncoproteins with peptide-centric CARs
a. SKNAS tumor cells co-cultured with A7 CAR shows potent killing. SKNAS cells plated on day 0 (top), non-targeting CAR (left) and A7 CAR (right) added after 18 h; measuring tumor confluence (green) and cleaved caspase (red) on day 2 (bottom) shows tumor outgrowth with non-specific CAR and killing of all tumor cells with A7 CAR. Representative images shown from experiment repeated three times using n = 3 replicates in each experiment. b. PHOX2B is expressed in neuroblastoma cell lines (SKNAS, SKNFI, and NBSD), and not in HLA-matched controls (SW620, KATO III, and HEPG2). PHOX2B is expressed in SW620 cells transduced with full-length PHOX2B. For gel source data from single experiment, see Supplementary Fig. 1.
