Extended Data Fig. 7: Epithelial IFN-γ signaling controls antigen presentation by IECs.
From: Epithelial IFNγ signalling and compartmentalized antigen presentation orchestrate gut immunity
Flow cytometric enumeration of MHCII (A and C) or MHCI (B) or SIINFEKL-MHCI complex (D-E) on the surface of the colonic IECs after infection with CitroWT (A and E) or CitroOva (B-D) at indicated day p.i. (A) or on day 12 p.i. (n = 5 per group). (B-E). Tamoxifen treatment was applied to induce the expression of the transgenes (B-E) at days 3-6 p.i. (F) Ifngr1fl/fl and Ifngr1fl/flVilCre mice were infected with Citrobacter and treated with anti-CD4 (n = 6) or isotype antibodies (n = 4) every 2 days starting at day 6 and sacrificed on day 12 p.i. The horizontal bar represents the median, and each symbol represents an individual mouse. Data were analyzed by (A) by ANOVA followed by Holm-Sidak post-test or (B-D and F) Kruskal-Wallis test followed by Dunn’s post-test or (E) Mann-Whitney U test (two-sided).
