Extended Data Fig. 8: Antigen presentation via MHCI by IECs promotes eATPase production from CD8ab+ IE-T cells.
From: Epithelial IFNγ signalling and compartmentalized antigen presentation orchestrate gut immunity
(A) Design and genotyping of B2mfl/fl mouse, representative of two independent lines. (B-K) B2mfl/fl and B2mfl/flVilCreERT2 mice were infected with ova-expressing Citrobacter on day 0, treated with tamoxifen on days 3–6 and sacrificed on day 12. Colonic IECs on day 12 were analyzed for (B) MHCI expression (n = 5 for B2mfl/fl and n = 7 B2mfl/flVilCreERT2) and (C) IE-T cells for CD39 expression (n = 5 per group) (D-E) (n = 3 for d0 and n = 5 for d6, d12 per group) by flow cytometry. (F-I) quantification of indicated analytes from colon explants (n = 8 per group) and (J) GM-CSF production from the ova-specific CD4+ IE-T cells and (K) Citrobacter colonization in the colon at day 12 p.i. (n = 5 per group). (L-N) B2mfl/fl and B2mfl/flVilCreERT2 mice were infected with ova-expressing Citrobacter on day 0, treated with tamoxifen on days 3–6, injected with anti-NK1.1 or isotype antibodies on days 2, 4, 6, 8 and 10 and sacrificed on day 12 (n = 5 per group). (L) IFN-γ production from NK1.1+ TCRαβ− TCRγδ− cells (M) CD45+CD49b+ cells and (N) colon length at day 12. The horizontal bar represents the median, and each symbol represents an individual mouse. Data were analyzed by (B, D, E and N) ANOVA test followed by Sidak’s post-test or (C, F-L) Kolmogorov-Smirnov test.
