Extended Data Fig. 9: Antigen presentation by IECs promotes eATPase production from CD4+ IE-T cells.
From: Epithelial IFNγ signalling and compartmentalized antigen presentation orchestrate gut immunity
H2abfl/fl and H2abfl/flVilCreERT2 mice were infected with ova-expressing Citrobacter on day 0, treated with tamoxifen on days 3-6, and sacrificed on day 12 (n = 5-8 per group). (A) Colonic IECs and myeloid cells were analyzed for MHCII expression on day 12 and (C-F) IE-T cells for CD39 expression by flow cytometry (n = 5 per group). (G) Entpd5 expression by RT-PCR after purification (n = 6 per group). (H-K) quantification of indicated analytes from colon explants (n = 8 pe group). (L-M) GM-CSF production from the CD4+ IE-T cells (n = 5 per group). (N) colon length and (O) Citrobacter colonization in the colon at day 12 p.i. (n = 8 per group). The horizontal bar represents the median, and each symbol represents an individual mouse. Data were analyzed by (A-O) Mann-Whitney U test (two-sided).
