Extended Data Fig. 1: Progressive increase in IFN-γ signaling and antigen presentation by IECs during colitis.
From: Epithelial IFNγ signalling and compartmentalized antigen presentation orchestrate gut immunity
C57BL/6 J mice were infected with Citrobacter rodentium. (A) Citrobacter colonization in the colon (n = 5 per group) and (B) quantification of Ifng transcript in colon tissue by RT-PCR (n = 4 for d0 and d21, n = 6 for d6 and n = 5 for d12). STAT1 pTyr701 analyses in IECs by (C) immunoblotting, representative of two independent experiments and (D) flow cytometry (n = 3 for d0, n = 6 for d6 and n = 5 for d12 and n = 4 for d21). (E) Quantification of Irf1 transcript in purified IECs (EpCAM+) by RT-PCR (n = 4 for d0, d6 and d21, n = 5 for d12). Flow cytometric analyses for (F and G) MHCI (n = 4 for d0, n = 5 for d6 and d12) and (H and I) MHCII (n = 4 for d0, n = 5 for d6 and d12) in the IECs from colons of mice at indicated day post-infection. (J) Colon histology score in Ifngr1fl/fl and Ifngr1fl/fl VilCre mice. The horizontal bar represents the median, and each symbol and each lane in the immunoblot represents an individual mouse. Data were analyzed by (A, B, D, E, G and I) Brown-Forsthye and Welsch ANOVA tests followed by the Dunnet’s post-hoc test.
