Extended Data Fig. 4: Epithelial sensing of IFN-γ restrains NLRP3 inflammasome activation in macrophages.
From: Epithelial IFNγ signalling and compartmentalized antigen presentation orchestrate gut immunity
(A-K) Ifngr1fl/fl and Ifngr1fl/fl VilCre mice were infected with Citrobacter rodentium. (A and B) Evaluation of indicated cytokines in colon explants (n = 4 for d0, d6 and n = 8 for d12, per group) and (C) immunoblotting for caspase-1 p10 in colon lysates, representative of two independent experiments. (D) Evaluation of IL-1α in colon explants, (E-F) Colon histology analyses and (G) Citrobacter colonization after isotype (n = 8), αβ-ATP (n = 8), apyrase (n = 6), MCC950 (n = 6) or anti-IL1R (n = 6) treatment. (H-K) Ifngr1fl/fl and Ifngr1fl/fl VilCre mice were infected and given 500ug of anti-CSF1R or isotype control antibody on days 7, 9 and 11 p.i and sacrificed on day 12 p.i. (n = 8 per group). (H) Flow cytometric plot for colon macrophages from Ifngr1fl/fl VilCre mice treated with anti-CSF1R or isotype control antibody. (I) Evaluation of indicated cytokines in colon explants. (J) Flow-cytometric analyses of CD4+ IE-T cells and GM-CSF production from them and (K) colon length at day 12 p.i. Data were analyzed by Brown-Forsythe and Welch’s ANOVA followed by Dunnett’s post-test. Scale bar = 100 um.
