Fig. 1: CD201⁺ fascia fibroblasts differentiate into specialized states during wound healing.

a, Uniform manifold approximation and projection (UMAP) analysis of all fibroblast clusters embedded with PAGA connectivities for trajectory inference. FB, fibroblast; MyoFB, myofibroblast; proinf., proinflammatory. b, Left, PAGA connectivity values with potential sources (rows) and fates (column) colour-coded as most (green), intermediate (yellow) and least probable (orange). Right, schematic of the potential trajectories highlighting the fascia-to-myofibroblast trajectory. c, UMAP analyses of fibroblasts from the fascia-to-myofibroblast trajectory, colour-coded for individual clusters (top) and velocity pseudotime score (bottom). d, Genetic lineage system to trace the fate of CD201⁺ fascia fibroblasts during wound healing. e, Representative histology of uninjured (left) Cd201^creERR26^Ai14 and 7 dpi wound (middle) immunolabelled for αSMA. Right, the contribution of CD201⁺ fibroblasts to the myofibroblast pool was determined for 6 wounds from 3 mice. Two-tailed Student’s t-tests. The dotted line delimits the fascia or wound region. Arrowheads indicate the original injury site. The inset shows an expanded view of the outlined region. f, Representative whole-mount immunostaining for the endothelial marker PECAM1 in uninjured Cd201^creERR26^Ai1 fascia. Arrows indicate labelled cells dispersed away from the adventitial space. g, Representative high-magnification images showing the expression of PDPN, pSTAT3 or RUNX2 in traced cells (left) and quantification at 3 and 7 dpi (right). Values obtained from at least three biological replicates. h, Genetic lineage system to trace the fate of proinflammatory fibroblast. i, Representative histology of uninjured skin immunolabelled for PDGFRα (left) and a 7-dpi wound (right) of Pdpn^creERR26^mTmG immunolabeled for αSMA. The dotted line delimits the fascia or wound region. Arrowheads indicate the original injury site. The inset shows an expanded view of the outlined region. Scale bars: 500 μm (f and e,i, main images) and 50 μm (g and e,i, expanded views).