Extended Data Fig. 6: Expression of wound fibroblast markers on the CD201CreERR26Ai14 and PdpnCreERR26mTmG genetic lineage tracing systems.
a. Representative low and high magnification (inserts) images showing the expression of PDPN (top), pSTAT3 (middle), and RUNX2 (bottom) in TdTomato-labelled fascia-derived cells on 3 (left) and 7 dpi (right) wounds. b. Representative low (left) and high magnification (right) images showing the expression of PDPN (top), pSTAT3 (middle), and RUNX2 (bottom) in GFP-labelled proinflammatory-derived cells on wounds at indicated timepoints. c. Representative low (left) and high magnification (middle) images showing the residual labelling with GFP in lymphatic vessels (LYVE1+) in wounds at indicated timepoints. Cell fractions of labelled-lymphatic endothelial cells from total traced and from total lymphatic vessels in wounds at indicated timepoints. N = 3 biological replicates. Dotted line delimits the wound region. Arrowheads indicate the original injury site. Scale bars: 500 microns in low magnification, and 50 microns in high magnification micrographs.
