Extended Data Fig. 1: Longitudinal single-cell RNA sequencing of stromal cells during murine wound healing.

a. Experimental approach for the Engrailed-1 lineage tracing coupled with stromal cell enrichment and Dropseq method for single-cell sequencing. b. UMAP representations of complete dataset color-coded for samples (time points and cell number, left) and Pdgfra expression (right) to identify fibroblasts. c. UMAP representation of fibroblast clusters (left) and the cluster composition of the different samples (right) revealing homeostatic (always present) and injury-specific populations (absent in uninjured sample). d. Fraction in % of each injury-specific population across samples (time-points). e. Ridge plots depicting profile scores from indicated fibroblast subtypes in each cluster. Gray lines indicate the overall mean for each profile score and the white lines indicate the cluster mean. Colour arrows indicate clusters with the highest profile for each fibroblast subtype. f. Gene expression dot-plots of top markers for homeostatic (left) and injury-related (right) clusters. g. Overrepresented GO terms in indicated injury cluster. Scatter plots show the fold enrichment (x-axis) of the term (y-axis), genes expressed/genes in term coverage in % (dot size), and the false discovery rate value (colour gradient).