Fig. 2: High-quality dataset for the exploration of the interactome.
From: The social and structural architecture of the yeast protein interactome
a, Clusters derived from our interactome for a range of challenging complexes such as chromatin-associated, soluble and membrane-bound complexes of various organelles. In each case, all known subunits were retrieved. The cell image was created with BioRender.com. b, Left, tag-based quantification enables retrieval of abundance information for the baits in a generic manner. Right, correlation of tag peptide-based signals with a literature compilation of yeast protein abundances18. c, Validation of selected clusters containing novel interactions or uncharacterized proteins using N-terminal GFP-tagged baits additional to the C-terminal tagged library used in the original screen. For the full validation panel, see Supplementary Fig. 1. d, For the non-taggable chaperonin containing t-complex (CCT), profile correlation analysis nevertheless reveals its subunits and interactors. Proteins with two and more interactions are shown. Interactions based on correlation only are shown in red (dashed) and previously unreported interactions with CCT are in green. e, The yeast-interactome web application (www.yeast-interactome.org) allows exploration of interaction data for interactions of interest. For all bait proteins, pull-downs are depicted as volcano plots together with a box plot that shows the mass spectrometry intensities of user-selected prey proteins. Subnetworks from pull-downs of the selected bait and reverse pull-downs or significant interactors can be displayed. Further features include full and subnetwork exploration, correlation visualization and an overview of sample quality.
