Extended Data Fig. 7: Sequence and structural analyses of pro-IL-18 recognition by inflammatory caspases.
From: Recognition and maturation of IL-18 by caspase-4 noncanonical inflammasome
a, Sequence alignment of the protease-domains of inflammatory caspases. Identical residues are in red background and conserved ones are in red. Numbers of starting residues are indicated on the left. Residues responsible for caspase-4 binding to pro-IL-18 are conserved in caspase-5 and -1 but differ in caspase-11 as colour-highlighted. b, A modelled structure of the caspase-1–pro-IL-18 complex. The structure of caspase-1-p20/p10 in the GSDMD-C complex (PDB code: 6KN0) was overlaid with that of caspase-4-p20/p10 in the pro-IL-18 complex. Close-up view on the right shows binding interfaces mediated by the exosites in caspase-4 and caspase-1. Residues that bind pro-IL-18 are shown as sticks.
