Extended Data Fig. 2: IL-18 processing induced by LPS-activated caspase-4 is defective in mice but effective in higher mammals.
From: Recognition and maturation of IL-18 by caspase-4 noncanonical inflammasome
a, Caspase-4 or caspase-11 was stably expressed in CASP4−/− (KO) HeLa cells. LDH release-based cell death data are shown as means (bars) of three replicates (circles). b, Caspase-11 was co-expressed with Flag-tagged human or mouse pro-IL-18 or mouse GSDMD in 293 T cells. a, b, Cells were stimulated with LPS electroporation. Cell supernatants (Sup.) (a) and lysates (a, b) were analysed by immunoblotting. c, d, LPS-primed primary (c) or immortalized (d) Gsdmd−/− (KO) BMDMs stably expressing mouse pro-IL-18-Flag (d) were stimulated with LPS electroporation to activate caspase-11 or LFn-BsaK plus protective antigen to activate the NAIP2-NLRC4 inflammasome. Cell lysates were analysed by immunoblotting. e, Pairs of caspase-4 and pro-IL-18 from human or other indicated mammals were co-expressed in 293 T cells. f, An indicated IL-1-family pro-cytokine with a C-terminal Flag tag was expressed in GSDMD−/− HeLa cells. e, f, Cells were stimulated with LPS electroporation, and cell lysates were analysed by immunoblotting. All data are representative of three independent experiments.
