Extended Data Fig. 3: Comparison of caspase-4, −1, and −11 activity in cleaving pro-IL-18 and effect of OspC3^ARD on caspase-4 processing of pro-IL-18.

a, Human or mouse pro-IL-18 was incubated with equivalent amounts of caspase-4/1-p20/p10 or caspase-11-p22/p10 for indicated times. b, Mouse pro-IL-18 was incubated with titrating relative molar amounts of caspase-11-p22/p10. Enz./Sub., enzyme/substrate. c, ITC measurements of the binding between caspase-4/11 and pro-IL-18. d, Human pro-IL-18 was incubated with caspase-4-p20/p10 alone or in the presence of excessive OspC3^ARD (40 times of caspase-4). a, b, d, Reaction mixtures were subjected to SDS-PAGE analyses. e, Solution or crystal samples of the OspC3^ARD–caspase-4-p20/p10-C/A–pro-IL-18 ternary complex were subjected to SDS-PAGE analyses. Data (a–d) are representative of three independent experiments.