Extended Data Fig. 6: scMultiomics data of male CaBl/BlCa WT and Msl2-KO NPCs.
(a) UMAPs of male CaBl (left) and BlCa (right) WT and Msl2-KO NPC single cell data based on three analytical strategies: independent RNA analysis (top), independent chromatin accessibility analysis (middle), and weighted nearest neighbour (WNN) analysis (bottom) representing a weighted combination of scRNA-seq and scATAC-seq modalities. The total numbers of cells analysed per condition are indicated in the figure. Cells are coloured by their sample names or by condition (WT vs. KO). (b) Pearson correlation of gene counts between bulk RNA-seq and scRNA-seq in male CaBl (left) and BlCa (right) WT and Msl2-KO NPCs. scRNA-seq gene counts were calculated by merging the total counts of all cells per gene. (c,d) Violin plots showing normalized counts of RNA expression and chromatin accessibility of genes from each category for individual alleles for male CaBl (c) and BlCa (d) WT (grey) and Msl2-KO NPCs (pink). Significance was scored by nonparametric Wilcoxon rank-sum test (two-sided), ****p < 0.0001, NS: p > 0.05. Exact p-values are summarized in the Source Data. Sample sizes for statistical tests are as follows: male CaBl NPCs (top to bottom): n = 177, 171, 67, 92, 1068, 300; male BlCa NPCs (top to bottom): n = 171, 148, 63, 39, 1501, 300. (e,f) Feature plots of representative bi-to-mono genes showing RNA expression (left) and chromatin accessibility (right) on WNN UMAPs for male CaBl (e) and BlCa (f) WT and Msl2-KO NPCs. Identical genes are shown for male reciprocal BlCa and CaBl NPCs.
