Extended Data Fig. 10: Conserved H3K27ac landscapes in human and mouse.
From: Conserved and divergent gene regulatory programs of the mammalian neocortex
a. UMAP embedding of Droplet Paired-Tag RNA profiles coloured by cell type in human M1 and mouse frontal cortex49. b. Heatmaps of human-biased and mammal level 3 (conserved patterns across all cell types) cCREs in human and mouse ordered by the cell type with the highest accessibility in human. Cell types with low coverage for H3K27ac were removed. c. A scatter plot highlighting the relationship between chromatin accessibility conservation, and H3k27ac conservation for each cCRE. Level 3 conserved human-mouse conserved H3K27ac elements are highlighted (N = 814). d. Scatter plots highlighting the relationship between H3K27ac conservation, and chromatin accessibility conservation at promoter-proximal elements (≤ 1 kb from a TSS, left), at promoter proximal-distal (>1 kb from a TSS, middle), and at chromatin accessibility mammal level 3 conserved cCREs (right). e. Box plots displaying H3K27ac signal (log2 CPM + 1, 4 kb genomic span) from the cell type with the highest signal for distal cCREs grouped by whether they are predicted to be enhancers or not by ABC model. H3K27ac counts were mappability normalized before converting to log2 CPM + 1. N = 281,840, 102,573; Box plots encompass 25th to 75th percentiles; central lines represent medians; whiskers represent 1.5 times the interquartile interval. Two-sided, unpaired Wilcoxon rank sum test for P-values. Species silhouettes in a and b created in BioRender.
