Fig. 5: Treatment-responsive glycosidic linkages, structures of their polysaccharide sources, cleavage sites and predicted products of CAZyme activity.
From: Bioactive glycans in a microbiome-directed food for children with malnutrition
a, Significant changes in faecal glycosidic linkage levels (q < 0.05) over time in upper- compared with lower-quartile WLZ responders. Probable polysaccharide sources for each of the 14 glycosidic linkages are noted in the middle (Supplementary Fig. 3). PULs present in P. copri MAGs Bg0018 and Bg0019 with known or predicted cleavage activity for the listed polysaccharide sources are noted on the right. b,c, The structures of the MDCF-2 polysaccharides galactomannan (b) and branched arabinan (c), plus glycan fragments and their constituent glycosidic linkages predicted to be liberated by PULs conserved between P. copri MAGs Bg0019 and Bg0018 (the results of PUL conservation analysis are shown in Fig. 4a). The arrows indicate putative sites of cleavage by CAZymes according to their known or predicted enzyme activities. The size of each arrow (large versus small) denotes the relative likelihood (high versus low, respectively) of glycosidic linkage cleavage by the indicated CAZymes, considering steric hindrance at glycan branch points.
