Extended Data Fig. 7: Wnt5b/Ror2 expressing clones suppress clonal and adjacent lef1 expression.

a-d, g-k, n-q. Embryos injected with the mRNA for the indicated constructs at the 8-cell stage in one blastomere to generate local clones were subjected to an in situ hybridization analysis against lef1 at 6 hpf. The embryos were stained and mounted with the animal pole up. Purple staining around the margin indicates the expression of lef1, and red staining (white star) indicates the site of the injected clonal cells. Clonal cells were co-injected with Mini-Emerald as a lineage tracer. Scale bar 200 µm. e,f,l,m,r,s. Classification of lef1 expression in the clonal cells and the receiving cells. The expression is classified according to the severity of the downregulation of the lef1 expression into not detectable (e.g., d), reduced (e.g., b), and normal (e.g., a). Numbers in the bars represent the percentage of total embryos in each group. (n = 31, 52, 79, 49, 24, 47, 24, 32, 91, 89, 16, 24, 24, n = numbers of embryos).