Extended Data Fig. 5: Tad2 proteins inhibit Thoeris mediated defense.
From: Phages overcome bacterial immunity via diverse anti-defence proteins
(a) Multiple sequence alignment of the original Tad2 from phage SPO1, and 5 Tad2 homologs that were chosen for experimental verification. Conserved residues are in purple. Black arrows indicate residues that are involved in 1″–3′ gcADPR binding. (b) Results of phage infection experiments with six phages of the SPO1 group. Data represent plaque-forming units per ml (PFU/ml) of phages infecting control cells (“no system”), cells expressing the Thoeris system (“Thoeris”), and cells co-expressing the Thoeris system and a Tad2 homolog. Shown is the average of three technical replicates, with individual data points overlaid.
