Extended Data Fig. 6: Tad2 binds 1″–3′ gcADPR.
From: Phages overcome bacterial immunity via diverse anti-defence proteins
(a) Incubation of Tad2 with 1″–3′ gcADPR in vitro does not yield observable degradation products. Representative HPLC traces of 1″–3′ gcADPR incubated with buffer, Tad2, or with the enzyme Cap-Clip known to cleave diphosphate linkages as a positive control. (b) Size-exclusion chromatography of 1″–3′ gcADPR-bound or apo state Tad2. 1″–3′ gcADPR-bound Tad2 shows a substantial shift compared to Tad2 in the apo state. (c) Surface plasmon resonance binding sensorgrams for Tad2 at five concentrations of 1″–3′ gcADPR. The black lines are the global fits using the instrument’s evaluation software. ka = 3.42E + 05 ± 5.2E + 02 (1/Ms), kd = 0.00798 ± 1E-05 (1/s). (d) Surface plasmon resonance binding sensorgrams for Tad2 at multiple concentrations of cADPR.
