Extended Data Fig. 9: Had1 proteins inhibit Hachiman-mediated defense.
From: Phages overcome bacterial immunity via diverse anti-defence proteins
(a) Results of phage infection experiments with eight phages of the SBSphiJ group. Data represent plaque-forming units per ml (PFU/ml) of phages infecting control cells (“no system”), cells expressing the Hachiman system (“Hachiman”), and cells co-expressing the Hachiman system and a Had1 homolog. Shown is the average of three technical replicates, with individual data points overlaid. (b) Structure-guided sequence alignment of Had1 homologs colored by BLOSUM62 score. (c) SDS-PAGE and (d) SEC-MALS analysis of purified Had1. Full-length Had1 elutes as a single species that is consistent with a homodimeric complex (predicted homodimer 12.5 kDa, observed 12.6 kDa). Data are representative of three independent experiments. For gel source data, see Supplementary Fig. 1.
