Extended Data Fig. 4: NMR data supporting the interaction of phosphorylated ARPP19 and FAM122A with PP2A:B55.
From: Cryo-EM structures of PP2A:B55–FAM122A and PP2A:B55–ARPP19
a. 2D [1H,15N] HSQC spectrum of 15N-labeled tpARPP19 alone (black) and in complex with PP2A:B55 (green). pS62 labeled for clarity. b. Peak intensity vs ARPP19 protein sequence plot for tpARPP19 alone (black) and when bound to PP2A:B55 (green). Secondary structure elements based on NMR CSI data are indicated. c. 2D [1H,15N] HSQC spectrum of 15N-labeled tpS62tpS104ARPP19 alone (black) and in complex with PP2A:B55 (green). pS62 and pS104 labeled for clarity. d. Peak intensity vs ARPP19 protein sequence plot for tpS62tpS104ARPP19 alone (black) and when bound to PP2A:B55 (green). Secondary structure elements based on NMR CSI data are indicated. e. 2D [1H,15N] HSQC spectrum of 15N-labeled FAM122ANterm alone (black) and in complex with PP2A:B55 (green). f. 2D [1H,15N] HSQC spectrum of 15N-labeled FAM122AID alone (black) and in complex with PP2A:B55 (green). g. Peak intensity vs FAM122AID protein sequence plot for FAM122AID alone (black) and when bound to PP2A:B55 (green). Secondary structure elements based on NMR CSI data are indicated.
