Extended Data Fig. 8: Impact of ferroptosis inhibitors on oxidant mediated liposomal rupture.
From: 7-Dehydrocholesterol is an endogenous suppressor of ferroptosis
a, Schematic representation of the CF/liposome assay used to monitor vesicle permeability. b, CF release from CF encapsulated liposomes generated using different sterols. CF release was stimulated using a mixture of iron and ascorbate (10 µM and 100 µM respectively). c, Impact of Lip1 on CF release from CF encapsulated liposomes containing cholesterol or 7-DHC. d, CF release in vesicles containing different ferroptosis inhibitors (10 µM) stimulated using a mixture of iron and ascorbate (20 µM and 200 µM respectively) in the absence (left panel) or in the presence of Ferrostatin-1 (Fer-1; right panel). Data are representative of mean ± s.d. of n = 3 technical replicates of 2 independent experiments.
