Extended Data Fig. 9: Role of truncated phospholipid in membrane permeability.
From: 7-Dehydrocholesterol is an endogenous suppressor of ferroptosis
a, Structure of selected truncated PC and related molecules tested. b Impact of native, peroxidised and truncated PC species in CF permeabilization. c, CF release in liposomes treated with different PC truncated species in a time and dose-dependent manner. d, Cell death induction by different PC truncated species in HT1080 cells in a time and dose-dependent manner. e, C50 of different truncated PL on CF release from liposomes containing 7-DHC or cholesterol. f, Schematic representation for the chemical basis of the PhotoPC probe: photoactivation leads to the generation of a defined mixture between the oxidized derivative PhotoOx-PC and the truncated derivative PhotoTrunc-PC. g, CF release in liposomes treated with PhotoPC before and after photoactivation in response to dose (fixed 1 h exposure) and time (fixed 365 µM). h, assessment of cell death (Draq7 positive) induced by equimolar concentrations of PhotoPC and PhotoTrunc-PC in HT1080 cells. Data are the mean ± s.d. of n = 6 wells of a 96-well plate from one representative of two independent experiments. i, Schematic representations of the events leading to the formation of truncated phospholipid species of formation of oxidatively truncated (phospho)lipid species and 7-DHC impact on it. Proferroptotic players are depicted in red and suppressing events are depicted in green.
