Fig. 2: Validation of synthetic enhancers in vivo.
From: Targeted design of synthetic enhancers for selected tissues in the Drosophila embryo
a, In vivo enhancer activity of one active sequence per tissue, as an example (for all other active sequences, see Extended Data Fig. 9). For each sequence, one representative embryo is shown from the total 200–300 embryos stained with double RNA fluorescence in situ hybridization (FISH). Scale bar, 100 μm. Predicted enhancer activity score and percentile value for the respective tissue model are shown. Top row, lacZ intensity reflects enhancer activity. Bottom row, lacZ intensity (green) overlaid with an endogenous marker gene (pink) for the respective tissue: elav (CNS), wg (epidermis), GATAe (gut), Mef2 (muscle) and tll (brain). The total numbers of active sequences per tissue are shown. b, Nucleotide contribution scores for the synthetic enhancers in a derived from the enhancer activity models for the respective tissues using DeepExplainer22,23,24. Instances of transcription factor motifs known to be associated with the respective tissues and predicted to be important for the enhancer activity are highlighted.
