Extended Data Fig. 6: Structure determination of the SlyB:BamA complex.
From: SlyB encapsulates outer membrane proteins in stress-induced lipid nanodomains
(a) Summary of the data processing strategies for the cryoEM 3D reconstruction of SlyB:BamA complexes (see Extended Data Fig. 1 for raw particles and representative 2D classes). Steps performed using Relion52 or cryoSPARC52 are coloured green or red, resp. Details of the processing procedures are described in the methods section. (b, c) Mask corrected Fourier Shell Correlation curves for the final 3D reconstructions of SlyB oligomer (b) and SlyB:BamA (c) complexes. Average map resolutions mentioned throughout the manuscript are according to the FSC 0.143 criterion (shown as dotted line). Data and model statistics for SlyB11 (PDB entry 7OJG) and SlyB13:BamA (PDB entry 7OJF) are found in Extanded Data Figure 10.
