Extended Data Fig. 4: Related to Fig. 2. Kupffer cells engulf live tumour cells in vivo and in vitro.
From: The nuclear factor ID3 endows macrophages with a potent anti-tumour activity
a- Schematic of time lapse intravital imaging of in vivo uptake of KPC-1-memtdT by KCs in liver from C57BL/6j mice 2 weeks after intra-portal injection of 1 × 106 KPC-1-memtdT cells in the presence of iv. injection of Tim4-AF647 antibodies and CellEvent Caspase-3/7 green reagent. Representative whole mount immunofluorescence imaging and time-lapse images are shown. Sequential images show KC1, KC2, KC3, KC4 engulfing tumour cells (yellow arrows), red arrow shows Cas3/7 green signalling. See Movie 1. b-Representative time-lapse images of in vitro uptake of KPC-1-memtdT by wide type KCs in Matrigel in the presence of F4/80-AF647 antibodies, and CellEvent Caspase-3/7 green reagent, and in the presence of Latrunculin-A or PBS control. In the PBS group, sequential images show a KC engulfing Cas3/7 green− tumour cells after 5 hrs of contact, between 12 and 14 hrs of culture (yellow arrows), and Cas3/7 green+ activation in cellular fragments ~6 hrs later (white arrows).
