Extended Data Fig. 6: Capsid spheres can complete NPC passage and enter the nuclei of mouse oocytes.

a, Labelled 40 nm capsid spheres were injected (along with a tetrameric tCherry injection marker) either into the cytoplasm or the nucleoplasm of fully grown mouse oocytes. Images show confocal laser scans 25 min after injection, when the endpoint distribution had already been reached. Note that the injection marker remained in the injected compartment, while cytoplasmically injected capsid spheres readily crossed the nuclear envelope and accumulated in intranuclear foci (indicated by white arrows in the zoomed-in images). The differences to Fig. 4c are (i) that complete oocytes were imaged here and not just smaller areas of interest and (ii) that the capsids were labelled with the FG-phobic sinGFP4a, which needs to be buried inside the capsid to avoid an arrest at the barrier (see Fig. 3b,d). Experiments were repeated independently with the same outcome (n = 3). b, Scheme of a fully grown mouse oocyte.