Extended Data Fig. 8: Preparation of 40 nm capsid spheres and CLPs with mCherry trapped inside.
From: HIV-1 capsids enter the FG phase of nuclear pores like a transport receptor
After the assembly of 40 nm capsid spheres and CLPs in the presence of excess amounts of mCherry, the particles were separated from unincorporated mCherry by gel filtration (Superose 6 10/300 column). Absorbance traces were recorded at 280 nm (total protein) and 587 nm (mCherry). a, Control experiment with free mCherry, showing no detectable aggregation (no void peak). b, Assembled 40 nm capsid spheres elute in the void volume, together with a fraction of the mCherry, indicating encapsulation. c, Assembled CLPs show a behaviour similar to b, with the encapsulated mCherry fraction co-eluting in the void peak.
