Fig. 5: CLPs act as cargo containers, partitioning an encapsulated client into a dense FG phase.
From: HIV-1 capsids enter the FG phase of nuclear pores like a transport receptor
a, A GLFG phase (as in Fig. 3) was probed for entry of free mCherry or of mCherry that had been encapsulated in 40 nm capsid spheres or in much larger (about 60–80 nm × 100–180 nm) CLPs (Fig. 1a). Scan settings were adjusted individually. Experiments were repeated independently with the same conclusion (n = 5). b, Quantification of a as in Fig. 3c,d. Note that CLP-encapsulated mCherry had an at least 10,000-fold higher partition coefficient than the firmly excluded free (non-encapsulated) mCherry. Numbers are means; bars indicate mean ± s.d. N = number of quantified FG particles and outside areas, respectively. Scale bar, 10 μm.
