Extended Data Fig. 1: BLI constructs.
From: HIV-1 capsids enter the FG phase of nuclear pores like a transport receptor
a, Purification of disulfide-stabilized CA hexamers (CA-P1A, A14C, E45C, W184A, M185A). Gel-filtration profile from a Superdex 200 (HR10/300) column with CA hexamers eluting as the major peak. The peak fraction was analysed by negative-stain EM. b, Illustration of the FG peptides used as BLI-probes in Fig. 2. The illustrations are drawn to scale. Vertical lines indicate individual FG- and FG-like dipeptides (including FG/S/T/A), the coloured sections with boundaries denote the actual FG-domain fragments tested, with the amino acid sequences also shown. Structured domains are depicted in grey. CypHD, cyclophilin-homology domain. c, SDS–PAGE analysis of all BLI-probes used in Fig. 2.
