Extended Data Fig. 2: BLI controls.
From: HIV-1 capsids enter the FG phase of nuclear pores like a transport receptor
a, Control BLI experiments showing that CA hexamer binds the established Nup153 and CPSF6 FG peptides. The CA-N57A mutant abolishes CPSF6 binding. Analyte concentrations of the dilution series are indicated. b, BLI-negative controls: Representative of FG probes, Nup58523–589 does not interact non-specifically with the analyte, as shown with importin α, which is not known to interact directly with FG peptides. Conversely, CA hexamers do not interact non-specifically with unrelated ligands, as shown with a Nesprin21423–1745 fragment. Both negative control experiments were performed with 5 µM analyte. c–e, SDS–PAGE analysis of BLI control probes.
