Extended Data Fig. 12: FISH of repeats to germline and somatic interphase nuclei.

Nuclei are labelled with the DNA stain NucBlue (blue) and for all panels labelled “no signal” fluorescence images are overexposed to both show background signal and aid in confirming the location of nuclei in those images. a,b, Germline enriched EEPs2 (red) and HFR10 (magenta), and the somatic repeat EEPs1 (green) are hybridized to nuclei isolated from a, germline: testes and b, soma: blood. c,d, Germline enriched repeats EEPs4 (red) and HFR5 (green), and the somatic repeat Soma3 (magenta) are hybridized to nuclei isolated from c, germline: testes and d, soma: blood. e,f, Germline enriched repeats HFR13 (magenta) and HFR6 (green), and the somatic repeat Soma1 (red) are hybridized to nuclei isolated from e, germline: testes and f, soma: blood. g,h, Germline enriched repeats EEEo2 (red) and HFR16 (magenta), and the somatic repeat EEPs1 (green) are hybridized to nuclei isolated from g, germline: testes and h, soma: blood. i,j, Germline enriched repeats HFR4 (red) and HFR8 (green), and the somatic repeat Soma3.1 (magenta) are hybridized to nuclei isolated from i, germline: testes and j, soma: blood. k,l, Germline enriched repeats EEPs2 (red) and EEPs3 (green), and the somatic repeat Soma3 (magenta) are hybridized to nuclei isolated from k, germline: testes and l, soma: blood. m,n, In situ Hybridization of probes for ten germline-enriched satellite sequences. m, Probes are hybridized to germline interphase nuclei. n, Probes are hybridized to germline interphase nuclei. The location of hybridization signals for telomere probes and approximate bounds of 18 germline-specific dyads, corresponding to nine distinct germline-specific chromosomes. For all images, pairs of repeats are shown to aid in visualizing the relative location of individual probes.