Extended Data Fig. 4: Overall and local resolution and angular distribution of 60S-UFM1-E3^UFM1 complexes.

(Local) resolution and angular distribution was assessed in RELION for the FLAG-UFM1 pulldown and the in vitro reconstituted sample (both resulting in State 3; see Extended Data Fig. 3) and the FLAG-UFL1 pulldown sample (yielding states 1, 2 and 3; see Extended Data Fig. 7). a, Gold-standard Fourier Shell Correlation (FSC) curves (obtained from RELION) for the reconstructions of the three states obtained from the UFL1 pulldown sample (states 1–3), the in vitro reconstituted sample (state 3) and the UFM1-pulldown sample (also state 3). CC = correlation corrected; UM = unmasked maps; MM = masked maps; PRMM = phase randomized masked maps. b, Cryo-EM maps, displayed after Gaussian low-pass filtering at a standard deviation of 2 in ChimeraX and colored according to local resolution. Shown are the entire 60S-UFM1-E3^UFM1 reconstructions (60S) as well as isolated densities for the E3^UFM1 (E3) and the SEC61 complex (SEC61; visualized in states 1 and 2 as obtained from the FLAG-UFL1 pulldown sample). c, Original (unfiltered) cryo-EM maps and angular distribution plots for final 60S-UFM1-E3^UFM1 complex reconstructions obtained from RELION. The height and color (from blue to red) of the cylinder bars is proportional to the number of particles in those views.