Extended Data Fig. 12: Depletion of NIPBL initiates a neutrophil-specific gene program in myeloid progenitors.
From: Nuclear morphology is shaped by loop-extrusion programs
a–f, Violin plots indicate the expression patterns of neutrophil-signature genes in GMPs (a), transitional neutrophils (b), myeloblasts/promyelocytes (c), myelocytes (d), immature neutrophils (e) and mature neutrophils (f) derived from ATTO-550− (control) and ATTO-550+ (NIPBL-depleted) populations. (t-test; ns: p > 0.05, *: p <= 0.05, **: p <= 0.01, ***: p <= 0.001, ****: p <= 0.0001). g, Model depicting the role of loop extrusion in mononuclear versus polymorphonuclear cell differentiation. Loop extrusion enforces the checkpoint that orchestrates mononuclear versus polymorphonuclear cell fate choice. Chromosomes in mononuclear cells are shown as rigid chromatin fibres (stacked rosettes)18. In polymorphonuclear cells, chromosomes are depleted for rosettes to adopt flexible conformations that enable the assembly of mega-loops and interchromosomal interactions. Illustration was created using BioRender.
