Fig. 3: Synthetic HPRT1 is active, whereas HPRT1R is inactive, in mouse ES cells.
From: Synthetic reversed sequences reveal default genomic states
a–c, Sequencing tracks for ATAC-seq, H3K4me3, RNAP2, H3K27ac and H3K27me3 CUT&RUN, and RNA-seq over the synthetic HPRT1 and HPRT1R loci integrated into the mouse genome. One clonal replicate is shown for each integration: HPRT1 chromosome X (2) (a), HPRT1R chromosome X (1) (b) and HPRT1R chromosome 3 (1) (c). Synthetic locus regions (HPRT1 and HPRT1R) are shaded. The HPRT1 coding sequence is indicated in a. Approximately 50 kb of flanking mouse genome is included, with annotated mouse genes indicated. RNA-seq tracks are stranded, displayed with reverse strand reads inverted and below forward strand reads. d, RNA-seq read counts for the synthetic loci (circles) and for 100-kb geneless regions of the mouse genome (squares, median of 70,107 100-kb sliding windows).
