Extended Data Fig. 8: Optic nerve sheath lymphatics drain the posterior eye.
From: Compartmentalized ocular lymphatic system mediates eye–brain immunity
a, Whole-mount murine optic nerve VEGFR3 staining and tracer signal on optic nerve 1 h later after AC (left) or IVT (right) injection. White arrowheads point to co-localization of VEGFR3 and tracer. b, c, Anti-LYVE1 antibody was IVT injected into the vitreous humor. Optic nerve sheaths were harvested 2 h later and co-stained with CD31, PROX1 and VEGFR3. d, Dye was IVT injected into the eye or intraventricularly into the brain. CSF was collected through the cisterna magna and measured using a fluorescent plate reader 1 h later after injection. e, Anti-LYVE1 antibody was injected IVT into the eye or ICM into the CSF. Dura was collected 2 h after injection and LYVE1 antibody binding on VEGFR3 positive lymphatic vessels was analyzed by immunofluorescence. Mice without anti-LYVE1 antibody injection were used as the control. f, AF647-OVA was IVT injected into the vitreous humor. Fluorescein was i.p. injected to label blood vessels. The kinetics of AF647-OVA drainage was tracked with Phoenix MICRON® IV imaging microscope. g, AF647-OVA was IVT injected into the vitreous humor. The eyes with optic nerve were harvested, and the location of AF647-OVA was analyzed at the indicated time points. h, Zoomed in images of g.
