Extended Data Fig. 9: Lymphatic inhibition enables repeated rAAV administrations.
From: Compartmentalized ocular lymphatic system mediates eye–brain immunity
a, Mice were IVT injected with rAAV-VEGFC, rAAV-sVEGFR3 or rAAV-control. Two months later, their optic nerve sheaths were harvested and stained with LYVE1. b, Similar to Fig. 4c, dCLNs were ligated before the primary rAAV-RFP injection. Their RFP intensity were quantified. c, Percentage of dye retention in the eye was measured for control, dCLN-ligated, or rAAV-sVEGFR3-injected mice. Data shown as mean ± s.e.m. P-values were calculated using a one-way ANOVA with multiple comparisons testing (Dunnett).
